2QDI

Drosophila OBP LUSH D118A mutation

2QDI の概要

• エントリーDOI: 10.2210/pdb2qdi/pdb
• 関連するPDBエントリー: 1OOF 1OOG 1OOH 1OOI 1T14 2GTE
• 分子名称: General odorant-binding protein lush, TRIETHYLENE GLYCOL (3 entities in total)
• 機能のキーワード: odorant binding protein, obp, pheromone binding protein, pbp, transport protein
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• 細胞内の位置: Secreted: O02372
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28643.34

構造登録者

Laughlin, J.D.,Jones, D.N.M. (登録日: 2007-06-20, 公開日: 2008-06-03, 最終更新日: 2024-11-20)

主引用文献

Laughlin, J.D.,Ha, T.S.,Jones, D.N.,Smith, D.P.
Activation of pheromone-sensitive neurons is mediated by conformational activation of pheromone-binding protein.
Cell(Cambridge,Mass.), 133:1255-1265, 2008

PubMed Abstract: Detection of volatile odorants by olfactory neurons is thought to result from direct activation of seven-transmembrane odorant receptors by odor molecules. Here, we show that detection of the Drosophila pheromone, 11-cis vaccenyl acetate (cVA), is instead mediated by pheromone-induced conformational shifts in the extracellular pheromone-binding protein, LUSH. We show that LUSH undergoes a pheromone-specific conformational change that triggers the firing of pheromone-sensitive neurons. Amino acid substitutions in LUSH that are predicted to reduce or enhance the conformational shift alter sensitivity to cVA as predicted in vivo. One substitution, LUSH(D118A), produces a dominant-active LUSH protein that stimulates T1 neurons through the neuronal receptor components Or67d and SNMP in the complete absence of pheromone. Structural analysis of LUSH(D118A) reveals that it closely resembles cVA-bound LUSH. Therefore, the pheromone-binding protein is an inactive, extracellular ligand converted by pheromone molecules into an activator of pheromone-sensitive neurons and reveals a distinct paradigm for detection of odorants.

PubMed: 18585358
DOI: 10.1016/j.cell.2008.04.046

実験手法

X-RAY DIFFRACTION (2 Å)