2QAD

Structure of tyrosine-sulfated 412d antibody complexed with HIV-1 YU2 gp120 and CD4

Summary for 2QAD

• Entry DOI: 10.2210/pdb2qad/pdb
• Descriptor: Envelope glycoprotein gp160, T-cell surface glycoprotein CD4, anti-HIV-1 antibody 412d light chain, ... (7 entities in total)
• Functional Keywords: viral protein/immune system, viral protein-immune system complex
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 8
• Total formula weight: 216737.58

Authors

Huang, C.-C.,Tang, M.,Robinson, J.,Wyatt, R.,Kwong, P.D. (deposition date: 2007-06-14, release date: 2007-09-25, Last modification date: 2024-11-20)

Primary citation

Huang, C.-C.,Lam, S.N.,Acharya, P.,Tang, M.,Xiang, S.-H.,Hussan, S.S.,Stanfield, R.L.,Robinson, J.,Sodroski, J.,Wilson, I.A.,Wyatt, R.,Bewley, C.A.,Kwong, P.D.
Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4
Science, 317:1930-1934, 2007

PubMed Abstract: The CCR5 co-receptor binds to the HIV-1 gp120 envelope glycoprotein and facilitates HIV-1 entry into cells. Its N terminus is tyrosine-sulfated, as are many antibodies that react with the co-receptor binding site on gp120. We applied nuclear magnetic resonance and crystallographic techniques to analyze the structure of the CCR5 N terminus and that of the tyrosine-sulfated antibody 412d in complex with gp120 and CD4. The conformations of tyrosine-sulfated regions of CCR5 (alpha-helix) and 412d (extended loop) are surprisingly different. Nonetheless, a critical sulfotyrosine on CCR5 and on 412d induces similar structural rearrangements in gp120. These results now provide a framework for understanding HIV-1 interactions with the CCR5 N terminus during viral entry and define a conserved site on gp120, whose recognition of sulfotyrosine engenders posttranslational mimicry by the immune system.

PubMed: 17901336
DOI: 10.1126/science.1145373

Experimental method

X-RAY DIFFRACTION (3.3 Å)