2Q8A

Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody

2Q8A の概要

• エントリーDOI: 10.2210/pdb2q8a/pdb
• 分子名称: Apical membrane antigen 1, 1F9 light chain, 1F9 heavy chain, ... (4 entities in total)
• 機能のキーワード: antigen-antibody complex, immune system
• 由来する生物種: Plasmodium falciparum; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 84461.29

構造登録者

Gupta, A.,Murphy, V.J.,Anders, R.F.,Batchelor, A.H. (登録日: 2007-06-10, 公開日: 2007-10-09, 最終更新日: 2024-10-09)

主引用文献

Coley, A.M.,Gupta, A.,Murphy, V.J.,Bai, T.,Kim, H.,Foley, M.,Anders, R.F.,Batchelor, A.H.
Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody.
PLoS Pathog., 3:1308-1319, 2007

PubMed Abstract: Identifying functionally critical regions of the malaria antigen AMA1 (apical membrane antigen 1) is necessary to understand the significance of the polymorphisms within this antigen for vaccine development. The crystal structure of AMA1 in complex with the Fab fragment of inhibitory monoclonal antibody 1F9 reveals that 1F9 binds to the AMA1 solvent-exposed hydrophobic trough, confirming its importance. 1F9 uses the heavy and light chain complementarity-determining regions (CDRs) to wrap around the polymorphic loops adjacent to the trough, but uses a ridge of framework residues to bind to the hydrophobic trough. The resulting 1F9-AMA1-combined buried surface of 2,470 A(2) is considerably larger than previously reported Fab-antigen interfaces. Mutations of polymorphic AMA1 residues within the 1F9 epitope disrupt 1F9 binding and dramatically reduce the binding of affinity-purified human antibodies. Moreover, 1F9 binding to AMA1 is competed by naturally acquired human antibodies, confirming that the 1F9 epitope is a frequent target of immunological attack.

PubMed: 17907804
DOI: 10.1371/journal.ppat.0030138

実験手法

X-RAY DIFFRACTION (2.4 Å)