2Q76

Mouse anti-hen egg white lysozyme antibody F10.6.6 Fab fragment

Summary for 2Q76

• Entry DOI: 10.2210/pdb2q76/pdb
• Related: 1MLB 1MLC 1P2C
• Descriptor: Fab F10.6.6 fragment Light Chain, Fab F10.6.6 fragment Heavy Chain (3 entities in total)
• Functional Keywords: antibody, fab fragment, lysozyme, affinity maturation, vh-vl interface., immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 4
• Total formula weight: 93038.87

Authors

Cauerhff, A.,Klinke, S.,Acierno, J.P.,Goldbaum, F.A.,Braden, B.C. (deposition date: 2007-06-06, release date: 2008-03-18, Last modification date: 2024-10-09)

Primary citation

Acierno, J.P.,Braden, B.C.,Klinke, S.,Goldbaum, F.A.,Cauerhff, A.
Affinity maturation increases the stability and plasticity of the Fv domain of anti-protein antibodies.
J.Mol.Biol., 374:130-146, 2007

PubMed Abstract: The somatic mutations accumulated in variable and framework regions of antibodies produce structural changes that increase the affinity towards the antigen. This implies conformational and non covalent bonding changes at the paratope, as well as possible quaternary structure changes and rearrangements at the V(H)-V(L) interface. The consequences of the affinity maturation on the stability of the Fv domain were studied in a system composed of two closely related antibodies, F10.6.6 and D44.1, which recognize the same hen egg-white lysozyme (HEL) epitope. The mAb F10.6.6 has an affinity constant 700 times higher than D44.1, due to a higher surface complementarity to HEL. The structure of the free form of the Fab F10.6.6 presented here allows a comparative study of the conformational changes produced upon binding to antigen. By means of structural comparison, kinetics and thermodynamics of binding and stability studies on Fab and Fv fragments of both antibodies, we have determined that the affinity maturation process of anti-protein antibodies affects the shape of the combining site and the secondary structure content of the variable domain, stabilizes the V(H)-V(L) interaction, and consequently produces an increase of the Fv domain stability, improving the binding to antigen.

PubMed: 17916365
DOI: 10.1016/j.jmb.2007.09.005

Experimental method

X-RAY DIFFRACTION (2 Å)