2Q3X

The RIM1alpha C2B domain

Summary for 2Q3X

• Entry DOI: 10.2210/pdb2q3x/pdb
• Descriptor: Regulating synaptic membrane exocytosis protein 1, SULFATE ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: c2 domain dimer, neurotransmitter release, transport protein
• Biological source: Rattus norvegicus (Norway rat)
• Cellular location: Cell membrane; Peripheral membrane protein: Q9JIR4
• Total number of polymer chains: 2
• Total formula weight: 38718.17

Authors

Guan, R.,Dai, H.,Tomchick, D.R.,Machius, M.,Sudhof, T.C.,Rizo, J. (deposition date: 2007-05-30, release date: 2007-08-28, Last modification date: 2024-10-30)

Primary citation

Guan, R.,Dai, H.,Tomchick, D.R.,Dulubova, I.,Machius, M.,Sudhof, T.C.,Rizo, J.
Crystal Structure of the RIM1alpha C(2)B Domain at 1.7 A Resolution.
Biochemistry, 46:8988-8998, 2007

PubMed Abstract: RIM proteins play critical roles in synaptic vesicle priming and diverse forms of presynaptic plasticity. The C-terminal C2B domain is the only module that is common to all RIMs but is only distantly related to well-studied C2 domains, and its three-dimensional structure and interactions have not been characterized in detail. Using NMR spectroscopy, we now show that N- and C-terminal extensions beyond the predicted C2B domain core sequence are necessary to form a folded, stable RIM1alpha C2B domain. We also find that the isolated RIM1alpha C2B domain is not sufficient for previously described protein-protein interactions involving the RIM1alpha C-terminus, suggesting that additional sequences adjacent to the C2B domain might be required for these interactions. However, analytical ultracentrifugation shows that the RIM1alpha C2B domain forms weak dimers in solution. The crystal structure of the RIM1alpha C2B domain dimer at 1.7 A resolution reveals that it forms a beta-sandwich characteristic of C2 domains and that the unique N- and C-terminal extensions form a small subdomain that packs against the beta-sandwich and mediates dimerization. Our results provide a structural basis to understand the function of RIM C2B domains and suggest that dimerization may be a crucial aspect of RIM function.

PubMed: 17630786
DOI: 10.1021/bi700698a

Experimental method

X-RAY DIFFRACTION (1.73 Å)