2PYR

PHOTOACTIVE YELLOW PROTEIN, 1 NANOSECOND INTERMEDIATE (287K)

2PYR の概要

• エントリーDOI: 10.2210/pdb2pyr/pdb
• 分子名称: PHOTOACTIVE YELLOW PROTEIN, 4'-HYDROXYCINNAMIC ACID (3 entities in total)
• 機能のキーワード: photoreceptor, light sensor for negative phototaxis
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14052.73

構造登録者

Perman, B.,Srajer, V.,Ren, Z.,Teng, T.Y.,Pradervand, C.,Ursby, T.,Bourgeois, D.,Schotte, F.,Wulff, M.,Kort, R.,Hellingwerf, K.,Moffat, K. (登録日: 1998-03-04, 公開日: 1999-04-06, 最終更新日: 2011-07-13)

主引用文献

Perman, B.,Srajer, V.,Ren, Z.,Teng, T.,Pradervand, C.,Ursby, T.,Bourgeois, D.,Schotte, F.,Wulff, M.,Kort, R.,Hellingwerf, K.,Moffat, K.
Energy transduction on the nanosecond time scale: early structural events in a xanthopsin photocycle.
Science, 279:1946-1950, 1998

PubMed Abstract: Photoactive yellow protein (PYP) is a member of the xanthopsin family of eubacterial blue-light photoreceptors. On absorption of light, PYP enters a photocycle that ultimately transduces the energy contained in a light signal into an altered biological response. Nanosecond time-resolved x-ray crystallography was used to determine the structure of the short-lived, red-shifted, intermediate state denoted [pR], which develops within 1 nanosecond after photoelectronic excitation of the chromophore of PYP by absorption of light. The resulting structural model demonstrates that the [pR] state possesses the cis conformation of the 4-hydroxyl cinnamic thioester chromophore, and that the process of trans to cis isomerization is accompanied by the specific formation of new hydrogen bonds that replace those broken upon excitation of the chromophore. Regions of flexibility that compose the chromophore-binding pocket serve to lower the activation energy barrier between the dark state, denoted pG, and [pR], and help initiate entrance into the photocycle. Direct structural evidence is provided for the initial processes of transduction of light energy, which ultimately translate into a physiological signal.

PubMed: 9506946
DOI: 10.1126/science.279.5358.1946

実験手法

X-RAY DIFFRACTION (1.9 Å)