2PXA

Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH and GTPG

Summary for 2PXA

• Entry DOI: 10.2210/pdb2pxa/pdb
• Related: 2PX2 2PX4 2PX5 2PX8 2PXC
• Descriptor: Genome polyprotein [Contains: Capsid protein C (Core protein); Envelope protein M (Matrix protein); Major envelope protein E; Non-structural protein 1 (NS1); Non-structural protein 2A (NS2A); Flavivirin protease NS2B regulatory subunit; Flavivirin protease NS3 catalytic subunit; Non-structural protein 4A (NS4A); Non-structural protein 4B (NS4B); RNA-directed RNA polymerase (EC 2.7.7.48) (NS5)], GUANOSINE-5'-TRIPHOSPHATE, S-ADENOSYL-L-HOMOCYSTEINE, ... (4 entities in total)
• Functional Keywords: murray valley encephalitis virus, methyltransferase, sah, gtpg, gtp, structural genomics, oxford protein production facility, oppf, transferase
• Biological source: Murray valley encephalitis virus (strain MVE-1-51)
• Cellular location: Capsid protein C: Virion (Potential). Peptide pr: Secreted (By similarity). Small envelope protein M: Virion membrane; Multi-pass membrane protein (By similarity). Envelope protein E: Virion membrane; Multi- pass membrane protein (By similarity). Non-structural protein 1: Secreted. Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease subunit NS2B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Non-structural protein 4A: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Non-structural protein 4B: Host endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). RNA-directed RNA polymerase NS5: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P05769
• Total number of polymer chains: 2
• Total formula weight: 62428.89

Authors

Assenberg, R.,Ren, J.,Verma, A.,Walter, T.S.,Alderton, D.,Hurrelbrink, R.J.,Fuller, S.D.,Owens, R.J.,Stuart, D.I.,Grimes, J.M.,Oxford Protein Production Facility (OPPF) (deposition date: 2007-05-14, release date: 2007-05-29, Last modification date: 2023-08-30)

Primary citation

Assenberg, R.,Ren, J.,Verma, A.,Walter, T.S.,Alderton, D.,Hurrelbrink, R.J.,Fuller, S.D.,Bressanelli, S.,Owens, R.J.,Stuart, D.I.,Grimes, J.M.
Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues.
J.Gen.Virol., 88:2228-2236, 2007

PubMed Abstract: We have determined the high resolution crystal structure of the methyltransferase domain of the NS5 polypeptide from the Murray Valley encephalitis virus. This domain is unusual in having both the N7 and 2'-O methyltransferase activity required for Cap 1 synthesis. We have also determined structures for complexes of this domain with nucleotides and cap analogues providing information on cap binding, based on which we suggest a model of how the sequential methylation of the N7 and 2'-O groups of the cap may be coordinated.

PubMed: 17622627
DOI: 10.1099/vir.0.82757-0

Experimental method

X-RAY DIFFRACTION (2.3 Å)