2PVX

NMR and X-ray Analysis of Structural Additivity in Metal Binding Site-Swapped Hybrids of Rubredoxin

Summary for 2PVX

• Entry DOI: 10.2210/pdb2pvx/pdb
• Related: 2PVE
• Descriptor: Rubredoxin, ZINC ION (3 entities in total)
• Functional Keywords: rubredoxin, chimeric, pyrococcus furiosus, electron transport
• Biological source: Pyrococcus furiosus
• Total number of polymer chains: 8
• Total formula weight: 49025.22

Authors

Wang, L.,LeMaster, D.M.,Hernandez, G.,Li, H. (deposition date: 2007-05-10, release date: 2007-12-18, Last modification date: 2023-08-30)

Primary citation

Lemaster, D.M.,Anderson, J.S.,Wang, L.,Guo, Y.,Li, H.,Hernandez, G.
NMR and X-ray analysis of structural additivity in metal binding site-swapped hybrids of rubredoxin
Bmc Struct.Biol., 7:81-81, 2007

PubMed Abstract: Chimeric hybrids derived from the rubredoxins of Pyrococcus furiosus (Pf) and Clostridium pasteurianum (Cp) provide a robust system for the characterization of protein conformational stability and dynamics in a differential mode. Interchange of the seven nonconserved residues of the metal binding site between the Pf and Cp rubredoxins yields a complementary pair of hybrids, for which the sum of the thermodynamic stabilities is equal to the sum for the parental proteins. Furthermore, the increase in amide hydrogen exchange rates for the hyperthermophile-derived metal binding site hybrid is faithfully mirrored by a corresponding decrease for the complementary hybrid that is derived from the less thermostable rubredoxin, indicating a degree of additivity in the conformational fluctuations that underlie these exchange reactions.

PubMed: 18053245
DOI: 10.1186/1472-6807-7-81

Experimental method

X-RAY DIFFRACTION (1.04 Å)