2PON

Solution structure of the Bcl-xL/Beclin-1 complex

2PON の概要

• エントリーDOI: 10.2210/pdb2pon/pdb
• 分子名称: Beclin-1, Apoptosis regulator Bcl-X (2 entities in total)
• 機能のキーワード: apoptosis; autophagy; bcl-2 family proteins; beclin-1, apoptosis inhibitor
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein: Q14457; Mitochondrion membrane; Single-pass membrane protein (By similarity): Q07817
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20398.60

構造登録者

Feng, W.,Huang, S.,Wu, H.,Zhang, M. (登録日: 2007-04-27, 公開日: 2007-09-04, 最終更新日: 2024-05-22)

主引用文献

Feng, W.,Huang, S.,Wu, H.,Zhang, M.
Molecular Basis of Bcl-xL's Target Recognition Versatility Revealed by the Structure of Bcl-xL in Complex with the BH3 Domain of Beclin-1.
J.Mol.Biol., 372:223-235, 2007

PubMed Abstract: Beclin-1, originally identified as a Bcl-2 binding protein, is an evolutionarily conserved protein required for autophagy. The direct interaction between Beclin-1 and Bcl-2 or Bcl-xL provides a potential convergence point for apoptosis and autophagy, two programmed cell death processes. Given the functional significance of the interaction between Beclin-1 and Bcl-2/Bcl-xL, we performed detailed biochemical and structural characterizations of this interaction. We demonstrated that the Bcl-xL-binding domain of Beclin-1 contains a BH3 domain. Therefore, Beclin-1 is a new member of the BH3-only family proteins. The structure of Bcl-xL in complex with the Beclin-1 BH3 domain was determined at high resolution by NMR spectroscopy. Although similar to other known BH3 domains, the Beclin-1 BH3 domain displays its own distinct features in the complex with Bcl-xL. Systematic analysis of all known Bcl-xL/BH3 domain complexes helped us to identify the molecular basis underlying the capacity of Bcl-xL to recognize diverse target sequences.

PubMed: 17659302
DOI: 10.1016/j.jmb.2007.06.069

実験手法

SOLUTION NMR