2PL7

Orhorhombic crystal structure of hydrophobin HFBII in the presence of a detergent

2PL7 の概要

• エントリーDOI: 10.2210/pdb2pl7/pdb
• 関連するPDBエントリー: 1R2M 2B97 2FZ6 2GVM 2PL4
• 分子名称: Hydrophobin-2, SULFATE ION, heptyl 1-thio-beta-D-glucopyranoside, ... (4 entities in total)
• 機能のキーワード: hydrophobin, amphiphile, protein surfactant, surface active protein
• 由来する生物種: Hypocrea jecorina
• 細胞内の位置: Spore wall: P79073
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14793.42

構造登録者

Kallio, J.M.,Rouvinen, J.P. (登録日: 2007-04-19, 公開日: 2007-07-17, 最終更新日: 2024-11-13)

主引用文献

Kallio, J.M.,Linder, M.B.,Rouvinen, J.
Crystal Structures of Hydrophobin HFBII in the Presence of Detergent Implicate the Formation of Fibrils and Monolayer Films.
J.Biol.Chem., 282:28733-28739, 2007

PubMed Abstract: Hydrophobins are small, amphiphilic proteins secreted by filamentous fungi. Their functionality arises from a patch of hydrophobic residues on the protein surface. Spontaneous self-assembly of hydrophobins leads to the formation of an amphiphilic layer that remarkably reduces the surface tension of water. We have determined by x-ray diffraction two new crystal structures of Trichoderma reesei hydrophobin HFBII in the presence of a detergent. The monoclinic crystal structure (2.2A resolution, R = 22, R(free) = 28) is composed of layers of hydrophobin molecules where the hydrophobic surface areas of the molecules are aligned within the layer. Viewed perpendicular to the aligned hydrophobic surface areas, the molecules in the layer pack together to form six-membered rings, thus leaving small pores in the layer. Similar packing has been observed in the atomic force microscopy images of the self-assembled layers of class II hydrophobin, indicating that the crystal structure resembles that of natural hydrophobin film. The orthorhombic crystal structure (1.0 A resolution, R = 13, R(free) = 15) is composed of fiber-like arrays of protein molecules. Rodlet structures have been observed on amphiphilic layers formed by class I hydrophobins; fibrils of class II hydrophobins appear by vigorous shaking. We propose that the structure of the fibrils and/or rodlets is similar to that observed in the crystal structure.

PubMed: 17636262
DOI: 10.1074/jbc.M704238200

実験手法

X-RAY DIFFRACTION (1 Å)