2PL4

D-(GGTATACC) under 0.55 GPa hydrostatic pressure

2PL4 の概要

• エントリーDOI: 10.2210/pdb2pl4/pdb
• 関連するPDBエントリー: 115D 1VJ4 2PKV 2PL8 2PLB 2PLO
• 分子名称: Synthetic DNA octamer (2 entities in total)
• 機能のキーワード: a dna, high-pressure, crystal stucture, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 4853.23

構造登録者

Prange, T.,Girard, E.,Kahn, R.,Fourme, R. (登録日: 2007-04-19, 公開日: 2007-07-24, 最終更新日: 2023-08-30)

主引用文献

Girard, E.,Prange, T.,Dhaussy, A.C.,Migianu-Griffoni, E.,Lecouvey, M.,Chervin, J.C.,Mezouar, M.,Kahn, R.,Fourme, R.
Adaptation of the base-paired double-helix molecular architecture to extreme pressure.
Nucleic Acids Res., 35:4800-4808, 2007

PubMed Abstract: The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage.

PubMed: 17617642
DOI: 10.1093/nar/gkm511

実験手法

X-RAY DIFFRACTION (1.65 Å)