2PJR

HELICASE PRODUCT COMPLEX

2PJR の概要

• エントリーDOI: 10.2210/pdb2pjr/pdb
• 分子名称: DNA (5'-D(*TP*TP*TP*TP*T)-3'), DNA (5'-D(*GP*C)-3'), DNA (5'-D(*AP*CP*TP*GP*C)-3'), ... (6 entities in total)
• 機能のキーワード: helicase pcra, hydrolase, dna, product complex, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 153362.23

構造登録者

Velankar, S.S.,Soultanas, P.,Dillingham, M.S.,Subramanya, H.S.,Wigley, D.B. (登録日: 1999-03-12, 公開日: 1999-04-08, 最終更新日: 2023-12-27)

主引用文献

Velankar, S.S.,Soultanas, P.,Dillingham, M.S.,Subramanya, H.S.,Wigley, D.B.
Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.
Cell(Cambridge,Mass.), 97:75-98, 1999

PubMed Abstract: We have determined two different structures of PcrA DNA helicase complexed with the same single strand tailed DNA duplex, providing snapshots of different steps on the catalytic pathway. One of the structures is of a complex with a nonhydrolyzable analog of ATP and is thus a "substrate" complex. The other structure contains a bound sulphate ion that sits in a position equivalent to that occupied by the phosphate ion produced after ATP hydrolysis, thereby mimicking a "product" complex. In both complexes, the protein is monomeric. Large and distinct conformational changes occur on binding DNA and the nucleotide cofactor. Taken together, these structures provide evidence against an "active rolling" model for helicase action but are instead consistent with an "inchworm" mechanism.

PubMed: 10199404
DOI: 10.1016/S0092-8674(00)80716-3

実験手法

X-RAY DIFFRACTION (2.9 Å)