2PIE

Crystal structure of the FHA domain of RNF8 in complex with its optimal phosphopeptide

2PIE の概要

• エントリーDOI: 10.2210/pdb2pie/pdb
• 関連するPDBエントリー: 2CSW
• 分子名称: E3 ubiquitin-protein ligase RNF8, phosphopeptide (3 entities in total)
• 機能のキーワード: fha domain, phosphopeptide, complex, ligase, signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : O76064
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16812.03

構造登録者

Grant, R.A.,Yaffe, M.B. (登録日: 2007-04-13, 公開日: 2007-12-11, 最終更新日: 2024-11-20)

主引用文献

Huen, M.S.,Grant, R.,Manke, I.,Minn, K.,Yu, X.,Yaffe, M.B.,Chen, J.
RNF8 Transduces the DNA-Damage Signal via Histone Ubiquitylation and Checkpoint Protein Assembly.
Cell(Cambridge,Mass.), 131:901-914, 2007

PubMed Abstract: DNA-damage signaling utilizes a multitude of posttranslational modifiers as molecular switches to regulate cell-cycle checkpoints, DNA repair, cellular senescence, and apoptosis. Here we show that RNF8, a FHA/RING domain-containing protein, plays a critical role in the early DNA-damage response. We have solved the X-ray crystal structure of the FHA domain structure at 1.35 A. We have shown that RNF8 facilitates the accumulation of checkpoint mediator proteins BRCA1 and 53BP1 to the damaged chromatin, on one hand through the phospho-dependent FHA domain-mediated binding of RNF8 to MDC1, on the other hand via its role in ubiquitylating H2AX and possibly other substrates at damage sites. Moreover, RNF8-depleted cells displayed a defective G2/M checkpoint and increased IR sensitivity. Together, our study implicates RNF8 as a novel DNA-damage-responsive protein that integrates protein phosphorylation and ubiquitylation signaling and plays a critical role in the cellular response to genotoxic stress.

PubMed: 18001825
DOI: 10.1016/j.cell.2007.09.041

実験手法

X-RAY DIFFRACTION (1.35 Å)