2PHK

THE CRYSTAL STRUCTURE OF A PHOSPHORYLASE KINASE PEPTIDE SUBSTRATE COMPLEX: KINASE SUBSTRATE RECOGNITION

2PHK の概要

• エントリーDOI: 10.2210/pdb2phk/pdb
• 分子名称: PHOSPHORYLASE KINASE, MC-PEPTIDE, MANGANESE (II) ION, ... (6 entities in total)
• 機能のキーワード: catalytic mechanism, dimerization, phosphorylase kinase, reversible phosphorylisation, substrate recognition, complex (transferase-peptide), complex (transferase-peptide) complex, complex (transferase/peptide)
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33632.05

構造登録者

Lowe, E.D.,Noble, M.E.M.,Skamnaki, V.T.,Oikonomakos, N.G.,Owen, D.J.,Johnson, L.N. (登録日: 1998-06-18, 公開日: 1999-01-13, 最終更新日: 2024-05-22)

主引用文献

Lowe, E.D.,Noble, M.E.,Skamnaki, V.T.,Oikonomakos, N.G.,Owen, D.J.,Johnson, L.N.
The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognition.
EMBO J., 16:6646-6658, 1997

PubMed Abstract: The structure of a truncated form of the gamma-subunit of phosphorylase kinase (PHKgammat) has been solved in a ternary complex with a non-hydrolysable ATP analogue (adenylyl imidodiphosphate, AMPPNP) and a heptapeptide substrate related in sequence to both the natural substrate and to the optimal peptide substrate. Kinetic characterization of the phosphotransfer reaction confirms the peptide to be a good substrate, and the structure allows identification of key features responsible for its high affinity. Unexpectedly, the substrate peptide forms a short anti-parallel beta-sheet with the kinase activation segment, the region which in other kinases plays an important role in regulation of enzyme activity. This anchoring of the main chain of the substrate peptide at a fixed distance from the gamma-phosphate of ATP explains the selectivity of PHK for serine/threonine over tyrosine as a substrate. The catalytic core of PHK exists as a dimer in crystals of the ternary complex, and the relevance of this phenomenon to its in vivo recognition of dimeric glycogen phosphorylase b is considered.

PubMed: 9362479
DOI: 10.1093/emboj/16.22.6646

実験手法

X-RAY DIFFRACTION (2.6 Å)