2PFV

S. cerevisiae Exo70 with additional residues to 2.1 Angrstrom resolution

2PFV の概要

• エントリーDOI: 10.2210/pdb2pfv/pdb
• 関連するPDBエントリー: 2B1E 2B7M
• 分子名称: Exocyst complex component EXO70 (2 entities in total)
• 機能のキーワード: helix-turn-helix, endocytosis-exocytosis complex, endocytosis/exocytosis
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle : P19658
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 64621.54

構造登録者

Moore, B.A.,Robinson, H.H.,Xu, Z. (登録日: 2007-04-05, 公開日: 2007-05-22, 最終更新日: 2024-02-21)

主引用文献

Moore, B.A.,Robinson, H.H.,Xu, Z.
The crystal structure of mouse Exo70 reveals unique features of the mammalian exocyst.
J.Mol.Biol., 371:410-421, 2007

PubMed Abstract: The exocyst is a eukaryotic tethering complex necessary for the fusion of exocytic vesicles with the plasma membrane. Its function in vivo is tightly regulated by interactions with multiple small GTPases. Exo70, one of the eight subunits of the exocyst, is important for the localization of the exocyst to the plasma membrane. It interacts with TC10 and Rho3 GTPases in mammals and yeast, respectively, and has been shown recently to bind to the actin-polymerization complex Arp2/3. Here, we present the crystal structure of Mus musculus Exo70 at 2.25 A resolution. Exo70 is composed of alpha-helices in a series of right-handed helix-turn-helix motifs organized into a long rod of length 170 A and width 35 A. Although the alpha-helical organization of this molecule is similar to that in Saccharomyces cerevisiae Exo70, major structural differences are observed on the surface of the molecule, at the domain boundaries, and in various loop structures. In particular, the C-terminal domain of M. musculus Exo70 adopts a new orientation relative to the N-terminal half not seen in S. cerevisiae Exo70 structures. Given the low level of sequence conservation within Exo70, this structure provides new insights into our understanding of many species-specific functions of the exocyst.

PubMed: 17583731
DOI: 10.1016/j.jmb.2007.05.018

実験手法

X-RAY DIFFRACTION (2.1 Å)