2PFF

Structural Insights of Yeast Fatty Acid Synthase

2PFF の概要

• エントリーDOI: 10.2210/pdb2pff/pdb
• 分子名称: Fatty acid synthase subunit alpha, Fatty acid synthase subunit beta, Tail protein (3 entities in total)
• 機能のキーワード: fatty acid synthase, acyl-carrier-protein, beta-ketoacyl reductase, beta-ketoacyl synthase, dehydratase, enoyl-[acyl-carrier-protein] reductase, acetyltransferase, malonyltransferase, transferase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 1104546.53

構造登録者

Xiong, Y.,Lomakin, I.B.,Steitz, T.A. (登録日: 2007-04-04, 公開日: 2007-07-10, 最終更新日: 2023-08-30)

主引用文献

Xiong, Y.,Lomakin, I.B.,Steitz, T.A.
Structural Insights of Yeast Fatty Acid Synthase
Cell(Cambridge,Mass.), 129:319-332, 2007

PubMed Abstract: In yeast, the whole metabolic pathway for making 16- and 18-carbon fatty acids is carried out by fatty acid synthase, a 2.6 megadalton molecular-weight macromolecular assembly containing six copies of all eight catalytic centers. We have determined its crystal structure, which illuminates how this enzyme is initially activated and then carries out multiple steps of synthesis in each of six sterically isolated reaction chambers. Six of the catalytic sites are in the wall of the assembly facing an acyl carrier protein (ACP) bound to the ketoacyl synthase domain. Two-dimensional diffusion of substrates to the catalytic sites may be achieved by the electrostatically negative ACP swinging to each of the six electrostatically positive catalytic sites. The phosphopantetheinyl transferase domain lies outside the shell of the assembly, inaccessible to ACP that lies inside, suggesting that the attachment of the pantetheine arm to ACP must occur before complete assembly of the complex.

PubMed: 17448991
DOI: 10.1016/j.cell.2007.03.013

実験手法

X-RAY DIFFRACTION (4 Å)