2PF0

F258I mutant of EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS at 1.9 A

2PF0 の概要

• エントリーDOI: 10.2210/pdb2pf0/pdb
• 関連するPDBエントリー: 1EQC 1EQP 2PB1 2PBO 2PC8
• 分子名称: Hypothetical protein XOG1 (2 entities in total)
• 機能のキーワード: exo-glucanase, candida albicans, carbohydrate binding, aromatic entranceway, hydrolase
• 由来する生物種: Candida albicans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 45758.38

構造登録者

Cutfield, S.M.,Cutfield, J.F.,Patrick, W.M. (登録日: 2007-04-03, 公開日: 2008-04-01, 最終更新日: 2024-10-30)

主引用文献

Patrick, W.M.,Nakatani, Y.,Cutfield, S.M.,Sharpe, M.L.,Ramsay, R.J.,Cutfield, J.F.
Carbohydrate binding sites in Candida albicans exo-beta-1,3-glucanase and the role of the Phe-Phe 'clamp' at the active site entrance.
Febs J., 277:4549-4561, 2010

PubMed Abstract: Candida albicans exo-β-1,3-glucanase (Exg; EC 3.2.1.58) is implicated in cell wall β-D-glucan remodelling through its glucosyl hydrolase and/or transglucosylase activities. A pair of antiparallel phenylalanyl residues (F144 and F258) flank the entrance to the active site pocket. Various Exg mutants were studied using steady-state kinetics and crystallography aiming to understand the roles played by these residues in positioning the β-1,3-D-glucan substrate. Mutations at the Phe-Phe entranceway demonstrated the requirement for double-sided CH/π interactions at the +1 subsite, and the necessity for phenylalanine rather than tyrosine or tryptophan. The Tyr-Tyr double mutations introduced ordered water molecules into the entranceway. A third Phe residue (F229) nearby was evaluated as a possible +2 subsite. The inactive double mutant E292S/F229A complexed with laminaritriose has provided the first picture of substrate binding to Exg and demonstrated how the Phe-Phe arrangement acts as a clamp at the +1 subsite. The terminal sugar at the -1 site showed displacement from the position of a monosaccharide analogue with interchange of water molecules and sugar hydroxyls. An unexpected additional glucose binding site, well removed from the active site, was revealed. This site may enable Exg to associate with the branched glucan structure of the C. albicans cell wall.

PubMed: 20875088
DOI: 10.1111/j.1742-4658.2010.07869.x

実験手法

X-RAY DIFFRACTION (1.9 Å)