2PC5

Native crystal structure analysis on Arabidopsis dUTPase

2PC5 の概要

• エントリーDOI: 10.2210/pdb2pc5/pdb
• 関連するPDBエントリー: 2P9O
• 分子名称: DUTP pyrophosphatase-like protein, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: dutpase, arabidopsis, magnesium, hydrolase
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 53607.73

構造登録者

Moriyama, H.,Bajaj, M. (登録日: 2007-03-29, 公開日: 2008-02-12, 最終更新日: 2023-08-30)

主引用文献

Bajaj, M.,Moriyama, H.
Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana.
Acta Crystallogr.,Sect.F, 63:409-411, 2007

PubMed Abstract: The deoxyuridine triphosphate nucleotidohydrolase gene from Arabidopsis thaliana was expressed and the gene product was purified. Crystallization was performed by the hanging-drop vapour-diffusion method at 298 K using 2 M ammonium sulfate as the precipitant. X-ray diffraction data were collected to 2.2 A resolution using Cu K alpha radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 69.90, b = 70.86 A, c = 75.55 A. Assuming the presence of a trimer in the asymmetric unit, the solvent content was 30%, with a V(M) of 1.8 A3 Da(-1).

PubMed: 17565183
DOI: 10.1107/S1744309107016004

実験手法

X-RAY DIFFRACTION (2.2 Å)