2P9S

Structure of bovine Arp2/3 complex co-crystallized with ATP/Mg2+

2P9S の概要

• エントリーDOI: 10.2210/pdb2p9s/pdb
• 関連するPDBエントリー: 2P9I 2P9K 2P9L 2P9N 2P9P 2P9U
• 分子名称: Actin-like protein 3, Actin-like protein 2, Actin-related protein 2/3 complex subunit 1B, ... (10 entities in total)
• 機能のキーワード: actin, wd repeat, complex, structural protein
• 由来する生物種: Bos taurus (cattle); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton (By similarity): P61157 A7MB62 Q58CQ2 Q3MHR7 Q3T035 Q148J6 Q3SYX9
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 225269.22

構造登録者

Nolen, B.J.,Pollard, T.D. (登録日: 2007-03-26, 公開日: 2007-05-29, 最終更新日: 2024-02-21)

主引用文献

Nolen, B.J.,Pollard, T.D.
Insights into the Influence of Nucleotides on Actin Family Proteins from Seven Structures of Arp2/3 Complex.
Mol.Cell, 26:449-457, 2007

PubMed Abstract: ATP is required for nucleation of actin filament branches by Arp2/3 complex, but the influence of ATP binding and hydrolysis are poorly understood. We determined crystal structures of bovine Arp2/3 complex cocrystallized with various bound adenine nucleotides and cations. Nucleotide binding favors closure of the nucleotide-binding cleft of Arp3, but no large-scale conformational changes in the complex. Thus, ATP binding does not directly activate Arp2/3 complex but is part of a network of interactions that contribute to nucleation. We compared nucleotide-induced conformational changes of residues lining the cleft in Arp3 and actin structures to construct a movie depicting the proposed ATPase cycle for the actin family. Chemical crosslinking stabilized subdomain 1 of Arp2, revealing new electron density for 69 residues in this subdomain. Steric clashes with Arp3 appear to be responsible for intrinsic disorder of subdomains 1 and 2 of Arp2 in inactive Arp2/3 complex.

PubMed: 17499050
DOI: 10.1016/j.molcel.2007.04.017

実験手法

X-RAY DIFFRACTION (2.68 Å)