2P7V

Crystal structure of the Escherichia coli regulator of sigma 70, Rsd, in complex with sigma 70 domain 4

2P7V の概要

• エントリーDOI: 10.2210/pdb2p7v/pdb
• 分子名称: Regulator of sigma D, RNA polymerase sigma factor rpoD, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: rsd, sigma 70, regulator of sigma 70, sigma 70 domain 4, transcription, regulation, helix-turn-helix, rsd-sigma 70 complex, sigma factor, escherichia coli rsd-sigma 70 complex, e. coli rsd-sigma 70 complex
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm (Potential): P0AFX4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26268.31

構造登録者

Patikoglou, G.A.,Westblade, L.F.,Campbell, E.A.,Lamour, V.,Lane, W.J.,Darst, S.A. (登録日: 2007-03-20, 公開日: 2007-08-21, 最終更新日: 2024-02-21)

主引用文献

Patikoglou, G.A.,Westblade, L.F.,Campbell, E.A.,Lamour, V.,Lane, W.J.,Darst, S.A.
Crystal structure of the Escherichia coli regulator of sigma70, Rsd, in complex with sigma70 domain 4.
J.Mol.Biol., 372:649-659, 2007

PubMed Abstract: The Escherichia coli Rsd protein binds tightly and specifically to the RNA polymerase (RNAP) sigma(70) factor. Rsd plays a role in alternative sigma factor-dependent transcription by biasing the competition between sigma(70) and alternative sigma factors for the available core RNAP. Here, we determined the 2.6 A-resolution X-ray crystal structure of Rsd bound to sigma(70) domain 4 (sigma(70)(4)), the primary determinant for Rsd binding within sigma(70). The structure reveals that Rsd binding interferes with the two primary functions of sigma(70)(4), core RNAP binding and promoter -35 element binding. Interestingly, the most highly conserved Rsd residues form a network of interactions through the middle of the Rsd structure that connect the sigma(70)(4)-binding surface with three cavities exposed on distant surfaces of Rsd, suggesting functional coupling between sigma(70)(4) binding and other binding surfaces of Rsd, either for other proteins or for as yet unknown small molecule effectors. These results provide a structural basis for understanding the role of Rsd, as well as its ortholog, AlgQ, a positive regulator of Pseudomonas aeruginosa virulence, in transcription regulation.

PubMed: 17681541
DOI: 10.1016/j.jmb.2007.06.081

実験手法

X-RAY DIFFRACTION (2.6 Å)