2OZR

MMP13 Catalytic Domain Complexed with 4-{[1-methyl-2,4-dioxo-6-(3-phenylprop-1-yn-1-yl)-1,4-dihydroquinazolin-3(2H)-yl]methyl}benzoic acid

Summary for 2OZR

• Entry DOI: 10.2210/pdb2ozr/pdb
• Related: 2ow9
• Descriptor: Collagenase 3, ZINC ION, CALCIUM ION, ... (6 entities in total)
• Functional Keywords: crystal complex structure, matrix metalloproteinase, mmp13 catalytic domain, mmp13 specific inhibitor, hydrolase
• Biological source: Homo sapiens (human)
• Cellular location: Secreted, extracellular space, extracellular matrix : P45452
• Total number of polymer chains: 8
• Total formula weight: 158258.78

Authors

Johnson, A.R.,Pavlovsky, A.G.,Ortwine, D.F.,Prior, F.,Man, C.-F.,Bornemeier, D.A.,Banotai, C.A.,Mueller, W.T.,McConnell, P.,Yan, C.H.,Baragi, V.,Lesch, C.,Roark, W.H.,Lie, J.J.,Fasquelle, V.,Wilson, M.,Robertson, D.,Datta, K.,Guzman, R.,Han, H.-K.,Dyer, R.D. (deposition date: 2007-02-27, release date: 2007-07-24, Last modification date: 2024-03-13)

Primary citation

Johnson, A.R.,Pavlovsky, A.G.,Ortwine, D.F.,Prior, F.,Man, C.F.,Bornemeier, D.A.,Banotai, C.A.,Mueller, W.T.,McConnell, P.,Yan, C.,Baragi, V.,Lesch, C.,Roark, W.H.,Wilson, M.,Datta, K.,Guzman, R.,Han, H.K.,Dyer, R.D.
Discovery and characterization of a novel inhibitor of matrix metalloprotease-13 that reduces cartilage damage in vivo without joint fibroplasia side effects.
J.Biol.Chem., 282:27781-27791, 2007

PubMed Abstract: Matrix metalloproteinase-13 (MMP13) is a Zn(2+)-dependent protease that catalyzes the cleavage of type II collagen, the main structural protein in articular cartilage. Excess MMP13 activity causes cartilage degradation in osteoarthritis, making this protease an attractive therapeutic target. However, clinically tested MMP inhibitors have been associated with a painful, joint-stiffening musculoskeletal side effect that may be due to their lack of selectivity. In our efforts to develop a disease-modifying osteoarthritis drug, we have discovered MMP13 inhibitors that differ greatly from previous MMP inhibitors; they do not bind to the catalytic zinc ion, they are noncompetitive with respect to substrate binding, and they show extreme selectivity for inhibiting MMP13. By structure-based drug design, we generated an orally active MMP13 inhibitor that effectively reduces cartilage damage in vivo and does not induce joint fibroplasias in a rat model of musculoskeletal syndrome side effects. Thus, highly selective inhibition of MMP13 in patients may overcome the major safety and efficacy challenges that have limited previously tested non-selective MMP inhibitors. MMP13 inhibitors such as the ones described here will help further define the role of this protease in arthritis and other diseases and may soon lead to drugs that safely halt cartilage damage in patients.

PubMed: 17623656
DOI: 10.1074/jbc.M703286200

Experimental method

X-RAY DIFFRACTION (2.3 Å)