2OZB

Structure of a human Prp31-15.5K-U4 snRNA complex

2OZB の概要

• エントリーDOI: 10.2210/pdb2ozb/pdb
• 関連するPDBエントリー: 1E7K
• 分子名称: RNA comprising the 5' Stem-Loop RNA of U4snRNA, U4/U6.U5 tri-snRNP 15.5 kDa protein, U4/U6 small nuclear ribonucleoprotein Prp31, ... (5 entities in total)
• 機能のキーワード: rna-protein complex, ribonucleoprotein particle (rnp), pre-mrna splicing, u4/u6 di-snrna, u4/u6 di-snrnp, hierarchical assembly, nop domain, rnp-binding domain, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus, nucleolus {ECO:0000269|PubMed:10593953, ECO:0000269|PubMed:12429849, ECO:0000269|Ref: P55769; Nucleus speckle: Q8WWY3
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 107806.16

構造登録者

Liu, S.,Luehrmann, R.,Wahl, M.C. (登録日: 2007-02-26, 公開日: 2007-03-20, 最終更新日: 2023-08-30)

主引用文献

Liu, S.,Li, P.,Dybkov, O.,Nottrott, S.,Hartmuth, K.,Luhrmann, R.,Carlomagno, T.,Wahl, M.C.
Binding of the human Prp31 Nop domain to a composite RNA-protein platform in U4 snRNP.
Science, 316:115-120, 2007

PubMed Abstract: Although highly homologous, the spliceosomal hPrp31 and the nucleolar Nop56 and Nop58 (Nop56/58) proteins recognize different ribonucleoprotein (RNP) particles. hPrp31 interacts with complexes containing the 15.5K protein and U4 or U4atac small nuclear RNA (snRNA), whereas Nop56/58 associate with 15.5K-box C/D small nucleolar RNA complexes. We present structural and biochemical analyses of hPrp31-15.5K-U4 snRNA complexes that show how the conserved Nop domain in hPrp31 maintains high RNP binding selectivity despite relaxed RNA sequence requirements. The Nop domain is a genuine RNP binding module, exhibiting RNA and protein binding surfaces. Yeast two-hybrid analyses suggest a link between retinitis pigmentosa and an aberrant hPrp31-hPrp6 interaction that blocks U4/U6-U5 tri-snRNP formation.

PubMed: 17412961
DOI: 10.1126/science.1137924

実験手法

X-RAY DIFFRACTION (2.6 Å)