2OY8

The crystal structure of OspA mutant

2OY8 の概要

• エントリーDOI: 10.2210/pdb2oy8/pdb
• 関連するPDBエントリー: 2OY1 2OY5 2OY7 2OYB
• 分子名称: Outer surface protein A (2 entities in total)
• 機能のキーワード: beta-sheet, membrane protein
• 由来する生物種: Borrelia burgdorferi (Lyme disease spirochete); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34518.45

構造登録者

Makabe, K.,Terechko, V.,Koide, A.,Koide, S. (登録日: 2007-02-21, 公開日: 2008-03-04, 最終更新日: 2023-08-30)

主引用文献

Biancalana, M.,Makabe, K.,Koide, A.,Koide, S.
Aromatic cross-strand ladders control the structure and stability of beta-rich peptide self-assembly mimics
J.Mol.Biol., 383:205-213, 2008

PubMed Abstract: Though beta-rich self-assemblies comprise a major structural class of polypeptides, a detailed understanding of the determinants of their structure and stability is lacking. In particular, the roles of repetitive stretches of side chains running the long axis of these beta-sheets, termed "cross-strand ladders," remain poorly characterized due to the inherently insoluble and heterogeneous nature of self-assemblies. To overcome these experimental challenges, we have established a complementary experimental system termed "peptide self-assembly mimics" (PSAMs). The PSAMs capture a defined number of self-assembly-like peptide repeats within a soluble beta-rich protein, making structural and energetic studies possible. In this work, we investigated the role of cross-strand ladders containing aromatic residues, which are prominent in self-assembling peptides. A combination of solution data and high-resolution crystal structures revealed that a single cross-strand ladder consisting solely of Tyr significantly stabilized, rigidified, and flattened the PSAM beta-sheet. These characteristics would stabilize each beta-sheet layer of a self-assembly and direct sheet conformations compatible with lamination. Our results therefore provide a rationale for the abundance of aromatic amino acids in fibril-forming peptides and establish important roles of cross-strand Tyr ladders in the structure and stability of beta-rich peptide self-assemblies.

PubMed: 18762191
DOI: 10.1016/j.jmb.2008.08.031

実験手法

X-RAY DIFFRACTION (2 Å)