2OXV

Structure of the A138T promiscuous mutant of the EcoRI restriction endonuclease bound to its cognate recognition site.

2OXV の概要

• エントリーDOI: 10.2210/pdb2oxv/pdb
• 分子名称: DNA (5'-D(*TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3'), Type II restriction enzyme EcoRI (3 entities in total)
• 機能のキーワード: ecori, type ii restriction endonuclease, protein-dna interactions, promiscuous mutant, relaxed specificity mutant, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35098.99

構造登録者

Sapienza, P.J.,Rosenberg, J.M.,Jen-Jacobson, L. (登録日: 2007-02-21, 公開日: 2007-10-23, 最終更新日: 2023-08-30)

• 主引用文献: Sapienza, P.J.,Rosenberg, J.M.,Jen-Jacobson, L.; Structural and thermodynamic basis for enhanced DNA binding by a promiscuous mutant EcoRI endonuclease.; Structure, 15:1368-1382, 2007; PubMed: 17997963; DOI: 10.1016/j.str.2007.09.014

実験手法

X-RAY DIFFRACTION (1.95 Å)