2OXM

Crystal structure of a UNG2/modified DNA complex that represent a stabilized short-lived extrahelical state in ezymatic DNA base flipping

2OXM の概要

• エントリーDOI: 10.2210/pdb2oxm/pdb
• 分子名称: DNA (5'-D(*TP*GP*TP*TP*AP*TP*CP*TP*T)-3'), DNA (5'-D(*AP*AP*AP*GP*AP*TP*(4MF)P*AP*CP*A)-3'), Uracil-DNA glycosylase, ... (4 entities in total)
• 機能のキーワード: enzyme dna complex, ung2, uracil dna glycosylase, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Isoform 1: Mitochondrion. Isoform 2: Nucleus: P13051
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 31322.05

構造登録者

Bianchet, M.A.,Krosky, D.J.,Stivers, J.T.,Amzel, L.M. (登録日: 2007-02-20, 公開日: 2007-10-30, 最終更新日: 2023-08-30)

主引用文献

Parker, J.B.,Bianchet, M.A.,Krosky, D.J.,Friedman, J.I.,Amzel, L.M.,Stivers, J.T.
Enzymatic capture of an extrahelical thymine in the search for uracil in DNA.
Nature, 449:433-437, 2007

PubMed Abstract: The enzyme uracil DNA glycosylase (UNG) excises unwanted uracil bases in the genome using an extrahelical base recognition mechanism. Efficient removal of uracil is essential for prevention of C-to-T transition mutations arising from cytosine deamination, cytotoxic U*A pairs arising from incorporation of dUTP in DNA, and for increasing immunoglobulin gene diversity during the acquired immune response. A central event in all of these UNG-mediated processes is the singling out of rare U*A or U*G base pairs in a background of approximately 10(9) T*A or C*G base pairs in the human genome. Here we establish for the human and Escherichia coli enzymes that discrimination of thymine and uracil is initiated by thermally induced opening of T*A and U*A base pairs and not by active participation of the enzyme. Thus, base-pair dynamics has a critical role in the genome-wide search for uracil, and may be involved in initial damage recognition by other DNA repair glycosylases.

PubMed: 17704764
DOI: 10.1038/nature06131

実験手法

X-RAY DIFFRACTION (2.5 Å)