2OVW

ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE

2OVW の概要

• エントリーDOI: 10.2210/pdb2ovw/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900005
• 分子名称: ENDOGLUCANASE I, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: glycosyl hydrolase, endoglucanase i, complexed with cellobiose, glycosylated protein, hydrolase
• 由来する生物種: Fusarium oxysporum
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 181880.13

構造登録者

Sulzenbacher, G.,Davies, G.J.,Schulein, M. (登録日: 1997-04-04, 公開日: 1998-04-08, 最終更新日: 2024-10-16)

主引用文献

Sulzenbacher, G.,Schulein, M.,Davies, G.J.
Structure of the endoglucanase I from Fusarium oxysporum: native, cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3 A resolution.
Biochemistry, 36:5902-5911, 1997

PubMed Abstract: The mechanisms involved in the enzymatic degradation of cellulose are of great ecological and commercial importance. The breakdown of cellulose by fungal species is performed by a consortium of free enzymes, known as cellobiohydrolases and endoglucanases, which are found in many of the 57 glycosyl hydrolase families. The structure of the endoglucanase I (EG I), found in glycosyl hydrolase family 7, from the thermophilic fungus Fusarium oxysporum has been solved at 2.3 A resolution. In addition to the native enzyme, structures have also been determined with both the affinity label, 3,4-epoxybutyl beta-D-cellobioside, and the reaction product cellobiose. The affinity label is covalently bound, as expected, to the catalytic nucleophile, Glu197, with clear evidence for binding of both the R and S stereoisomers. Cellobiose is found bound to the -2 and -1 subsites of the enzyme. In marked contrast to the structure of EG I with a nonhydrolyzable thiosaccharide analog, which spanned the -2, -1, and +1 subsites and which had a skew-boat conformation for the -1 subsite sugar [Sulzenbacher, G., et al. (1996) Biochemistry 35, 15280-15287], the cellobiose complex shows no pyranoside ring distortion in the -1 subsite, implying that strain is induced primarily by the additional +1 subsite interactions and that the product is found, as expected, in its unstrained conformation.

PubMed: 9153432
DOI: 10.1021/bi962963+

実験手法

X-RAY DIFFRACTION (2.3 Å)