2OSY

Endo-glycoceramidase II from Rhodococcus sp.: Lactosyl-Enzyme Intermediate

2OSY の概要

• エントリーDOI: 10.2210/pdb2osy/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900008
• 分子名称: Endoglycoceramidase II, beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: (alpha/beta)8 (tim) barrel, hydrolase
• 由来する生物種: Rhodococcus sp.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 104859.57

構造登録者

Caines, M.E.C.,Strynadka, N.C.J. (登録日: 2007-02-06, 公開日: 2007-02-27, 最終更新日: 2024-10-30)

主引用文献

Caines, M.E.,Vaughan, M.D.,Tarling, C.A.,Hancock, S.M.,Warren, R.A.,Withers, S.G.,Strynadka, N.C.
Structural and Mechanistic Analyses of endo-Glycoceramidase II, a Membrane-associated Family 5 Glycosidase in the Apo and GM3 Ganglioside-bound Forms.
J.Biol.Chem., 282:14300-14308, 2007

PubMed Abstract: endo-Glycoceramidase, a membrane-associated family 5 glycosidase, deviates from the typical polysaccharide substrate specificity of other soluble members of the family, preferentially hydrolyzing glycosidic linkages between the oligosaccharide and ceramide moieties of gangliosides. Here we report the first x-ray crystal structures of an endo-glycoceramidase from Rhodococcus sp., in the apo form, in complex with the ganglioside G(M3) (Svennerholm ganglioside nomenclature (Svennerholm, L. (1964) J. Lipid Res. 5, 145-155)), and trapped as a glycosyl-enzyme intermediate. These snapshots provide the first molecular insight into enzyme recognition and association with gangliosides, revealing the structural adaptations necessary for glycosidase-catalyzed hydrolysis and detailing a novel ganglioside binding topology. Consistent with the chemical duality of the substrate, the active site of endo-glycoceramidase is split into a wide, polar cavity to bind the polyhydroxylated oligosaccharide moiety and a narrow, hydrophobic tunnel to bind the ceramide lipid chains. The specific interactions with the ceramide polar head group manifest a surprising aglycone specificity, an observation substantiated by our kinetic analyses. Collectively, the reported structural and kinetic data provide insight toward rational redesign of the synthetic glycosynthase mutant of endo-glycoceramidase to enable facile synthesis of nonnatural, therapeutically useful gangliosides.

PubMed: 17329247
DOI: 10.1074/jbc.M611455200

実験手法

X-RAY DIFFRACTION (2.1 Å)