2OSE

Crystal Structure of the Mimivirus Cyclophilin

2OSE の概要

• エントリーDOI: 10.2210/pdb2ose/pdb
• 分子名称: Probable peptidyl-prolyl cis-trans isomerase, CHLORIDE ION (3 entities in total)
• 機能のキーワード: mimivirus, cyclophilin, isomerase
• 由来する生物種: Mimivirus
• 細胞内の位置: Virion : Q5UP71
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26641.77

構造登録者

Eisenmesser, E.Z.,Thai, V.,Renesto, P.,Raoult, D. (登録日: 2007-02-05, 公開日: 2007-12-18, 最終更新日: 2023-08-30)

主引用文献

Thai, V.,Renesto, P.,Fowler, C.A.,Brown, D.J.,Davis, T.,Gu, W.,Pollock, D.D.,Kern, D.,Raoult, D.,Eisenmesser, E.Z.
Structural, biochemical, and in vivo characterization of the first virally encoded cyclophilin from the Mimivirus.
J.Mol.Biol., 378:71-86, 2008

PubMed Abstract: Although multiple viruses utilize host cell cyclophilins, including severe acute respiratory syndrome (SARS) and human immunodeficiency virus type-1(HIV-1), their role in infection is poorly understood. To help elucidate these roles, we have characterized the first virally encoded cyclophilin (mimicyp) derived from the largest virus discovered to date (the Mimivirus) that is also a causative agent of pneumonia in humans. Mimicyp adopts a typical cyclophilin-fold, yet it also forms trimers unlike any previously characterized homologue. Strikingly, immunofluorescence assays reveal that mimicyp localizes to the surface of the mature virion, as recently proposed for several viruses that recruit host cell cyclophilins such as SARS and HIV-1. Additionally mimicyp lacks peptidyl-prolyl isomerase activity in contrast to human cyclophilins. Thus, this study suggests that cyclophilins, whether recruited from host cells (i.e. HIV-1 and SARS) or virally encoded (i.e. Mimivirus), are localized on viral surfaces for at least a subset of viruses.

PubMed: 18342330
DOI: 10.1016/j.jmb.2007.08.051

実験手法

X-RAY DIFFRACTION (2.04 Å)