2OR1

RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION

2OR1 の概要

• エントリーDOI: 10.2210/pdb2or1/pdb
• 分子名称: DNA (5'-D(*AP*AP*GP*TP*AP*CP*AP*AP*AP*CP*TP*TP*TP*CP*TP*TP*G P*TP*AP*T)-3'), DNA (5'-D(*TP*AP*TP*AP*CP*AP*AP*GP*AP*AP*AP*GP*TP*TP*TP*GP*T P*AP*CP*T)-3'), 434 REPRESSOR, ... (4 entities in total)
• 機能のキーワード: protein-dna complex, double helix, gene regulation-dna complex, gene regulation/dna
• 由来する生物種: Phage 434
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 27384.10

構造登録者

Aggarwal, A.K.,Rodgers, D.W.,Drottar, M.,Ptashne, M.,Harrison, S.C. (登録日: 1989-09-05, 公開日: 1989-09-05, 最終更新日: 2024-02-21)

主引用文献

Aggarwal, A.K.,Rodgers, D.W.,Drottar, M.,Ptashne, M.,Harrison, S.C.
Recognition of a DNA operator by the repressor of phage 434: a view at high resolution.
Science, 242:899-907, 1988

PubMed Abstract: The repressors of temperate bacteriophages such as 434 and lambda control transcription by binding to a set of DNA operator sites. The different affinity of repressor for each of these sites ensures efficient regulation. High-resolution x-ray crystallography was used to study the DNA-binding domain of phage 434 repressor in complex with a synthetic DNA operator. The structure shows recognition of the operator by direct interactions with base pairs in the major groove, combined with the sequence-dependent ability of DNA to adopt the required conformation on binding repressor. In particular, a network of three-centered bifurcated hydrogen bonds among base pairs in the operator helps explain why 434 repressor prefers certain sites over others. These bonds, which stabilize the conformation of the bound DNA, can form only with certain sequences.

PubMed: 3187531

実験手法

X-RAY DIFFRACTION (2.5 Å)