2OQU

High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths

2OQU の概要

• エントリーDOI: 10.2210/pdb2oqu/pdb
• 関連するPDBエントリー: 2OQN
• 分子名称: Elastase-1, CALCIUM ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: high pressure cryocooling, xenon, sad phasing, hydrolase
• 由来する生物種: Sus scrofa (pig)
• 細胞内の位置: Secreted: P00772
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26196.45

構造登録者

Kim, C.U.,Hao, Q.,Gruner, S.M. (登録日: 2007-02-01, 公開日: 2007-05-01, 最終更新日: 2024-11-13)

主引用文献

Kim, C.U.,Hao, Q.,Gruner, S.M.
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths.
Acta Crystallogr.,Sect.D, 63:653-659, 2007

PubMed Abstract: Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.

PubMed: 17452791
DOI: 10.1107/S0907444907011924

実験手法

X-RAY DIFFRACTION (1.8 Å)