2ONG

Crystal Structure of of limonene synthase with 2-fluorogeranyl diphosphate (FGPP) ezymatically converted to 2-fluorolinalyl diphosphate (FLPP)

2ONG の概要

• エントリーDOI: 10.2210/pdb2ong/pdb
• 関連するPDBエントリー: 2ONH
• 分子名称: 4S-limonene synthase, MANGANESE (II) ION, (3S)-2-fluoro-3,7-dimethylocta-1,6-dien-3-yl trihydrogen diphosphate, ... (5 entities in total)
• 機能のキーワード: monoterpene synthase, monoterpene cyclase, geranyl diphosphate, 2 fluorogeranyl diphosphate, linalyl diphosphate, 2-fluorolinalyl diphosphate, lyase
• 由来する生物種: Mentha spicata (spearmint)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 129621.14

構造登録者

Hyatt, D.C.,Youn, B.,Croteau, R.,Kang, C. (登録日: 2007-01-23, 公開日: 2007-03-06, 最終更新日: 2025-10-01)

主引用文献

Hyatt, D.C.,Youn, B.,Zhao, Y.,Santhamma, B.,Coates, R.M.,Croteau, R.B.,Kang, C.
Structure of limonene synthase, a simple model for terpenoid cyclase catalysis.
Proc.Natl.Acad.Sci.Usa, 104:5360-5365, 2007

PubMed Abstract: The crystal structure of (4S)-limonene synthase from Mentha spic ata, a metal ion-dependent monoterpene cyclase that catalyzes the coupled isomerization and cyclization of geranyl diphosphate, is reported at 2.7-A; resolution in two forms liganded to the substrate and intermediate analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain interactions in the homodimer and for changes in diphosphate-metal ion coordination and substrate binding conformation in the course of the multistep reaction.

PubMed: 17372193
DOI: 10.1073/pnas.0700915104

実験手法

X-RAY DIFFRACTION (2.7 Å)