2OKG

Structure of effector binding domain of central glycolytic gene regulator (CggR) from B. subtilis

2OKG の概要

• エントリーDOI: 10.2210/pdb2okg/pdb
• 分子名称: Central glycolytic gene regulator, CHLORIDE ION, GLYCERALDEHYDE-3-PHOSPHATE, ... (4 entities in total)
• 機能のキーワード: alpha/beta/alpha sandwich, rossmann-like fold, structural genomics, psi-2, protein structure initiative, midwest center for structural genomics, mcsg, transcription
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56147.75

構造登録者

Rezacova, P.,Moy, S.F.,Joachimiak, A.,Otwinowski, Z.,Midwest Center for Structural Genomics (MCSG) (登録日: 2007-01-16, 公開日: 2007-01-30, 最終更新日: 2024-10-16)

主引用文献

Rezacova, P.,Kozisek, M.,Moy, S.F.,Sieglova, I.,Joachimiak, A.,Machius, M.,Otwinowski, Z.
Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates.
Mol.Microbiol., 69:895-910, 2008

PubMed Abstract: Expression of genes in the gapA operon encoding five enzymes for triose phosphate interconversion in Bacillus subtilis is negatively regulated by the Central glycolytic genes Regulator (CggR). CggR belongs to the large SorC/DeoR family of prokaryotic transcriptional regulators, characterized by an N-terminal DNA-binding domain and a large C-terminal effector-binding domain. When no glucose is present in growth media, CggR binds to its target DNA sequence and blocks the transcription of genes in the gapA operon. In the presence of glucose, binding of the known effector molecule fructose-1,6-bisphosphate abolishes this interaction. We have identified dihydroxyacetone phosphate, glucose-6-phosphate and fructose-6-phosphate as additional CggR ligands that can bind to the effector-binding site. Crystal structures of C-CggR, the C-terminal effector-binding domain of CggR, both unliganded as well as in complex with the four ligands at resolutions between 1.65 and 1.80 A reveal unique ligand-specific structural changes in the binding site that affect the dimer interface. Binding affinities of these ligands were determined by isothermal titration calorimetry. Chemical cross-linking shows that CggR oligomerization is mediated through its effector-binding domain, and that binding of the different ligands differentially affects the distribution of oligomers. Electrophoretic mobility shift assays (EMSAs) confirmed a destabilizing effect of fructose-1,6-bisphosphate on the CggR/DNA complex, and also showed similar effects for dihydroxyacetone phosphate. Our results suggest that CggR stability and function may be modulated by various effectors in a complex fashion.

PubMed: 18554327
DOI: 10.1111/j.1365-2958.2008.06318.x

実験手法

X-RAY DIFFRACTION (1.65 Å)