2OIU

L1 Ribozyme Ligase circular adduct

Summary for 2OIU

Entry DOI	10.2210/pdb2oiu/pdb
Descriptor	L1 Ribozyme RNA Ligase, MAGNESIUM ION (3 entities in total)
Functional Keywords	ligase, ribozyme, in vitro selection, rna, active site
Total number of polymer chains	2
Total formula weight	46191.69
Authors	Robertson, M.P.,Scott, W.G. (deposition date: 2007-01-11, release date: 2007-03-13, Last modification date: 2023-12-27)
Primary citation	Robertson, M.P.,Scott, W.G. The structural basis of ribozyme-catalyzed RNA assembly. Science, 315:1549-1553, 2007 Cited by PubMed Abstract: Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly. PubMed: 17363667 DOI: 10.1126/science.1136231 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.6 Å)

Structure validation