2OIU

L1 Ribozyme Ligase circular adduct

2OIU の概要

• エントリーDOI: 10.2210/pdb2oiu/pdb
• 分子名称: L1 Ribozyme RNA Ligase, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: ligase, ribozyme, in vitro selection, rna, active site
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46191.69

構造登録者

Robertson, M.P.,Scott, W.G. (登録日: 2007-01-11, 公開日: 2007-03-13, 最終更新日: 2023-12-27)

主引用文献

Robertson, M.P.,Scott, W.G.
The structural basis of ribozyme-catalyzed RNA assembly.
Science, 315:1549-1553, 2007

PubMed Abstract: Life originated, according to the RNA World hypothesis, from self-replicating ribozymes that catalyzed ligation of RNA fragments. We have solved the 2.6 angstrom crystal structure of a ligase ribozyme that catalyzes regiospecific formation of a 5' to 3' phosphodiester bond between the 5'-triphosphate and the 3'-hydroxyl termini of two RNA fragments. Invariant residues form tertiary contacts that stabilize a flexible stem of the ribozyme at the ligation site, where an essential magnesium ion coordinates three phosphates. The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly.

PubMed: 17363667
DOI: 10.1126/science.1136231

実験手法

X-RAY DIFFRACTION (2.6 Å)