2OF6

Structure of immature West Nile virus

Summary for 2OF6

• Entry DOI: 10.2210/pdb2of6/pdb
• EMDB information: 1314
• Descriptor: envelope glycoprotein E (1 entity in total)
• Functional Keywords: em immature flavivirus west nile, icosahedral virus, virus
• Biological source: West Nile virus
• Total number of polymer chains: 3
• Total formula weight: 129816.29

Authors

Zhang, Y.,Kaufmann, B.,Chipman, P.R.,Kuhn, R.J.,Rossmann, M.G. (deposition date: 2007-01-02, release date: 2007-04-03, Last modification date: 2023-12-27)

Primary citation

Zhang, Y.,Kaufmann, B.,Chipman, P.R.,Kuhn, R.J.,Rossmann, M.G.
Structure of immature west nile virus.
J.Virol., 81:6141-6145, 2007

PubMed Abstract: The structure of immature West Nile virus particles, propagated in the presence of ammonium chloride to block virus maturation in the low-pH environment of the trans-Golgi network, was determined by cryo-electron microscopy (cryo-EM). The structure of these particles was similar to that of immature West Nile virus particles found as a minor component of mature virus samples (naturally occurring immature particles [NOIPs]). The structures of mature infectious flaviviruses are radically different from those of the immature particles. The similarity of the ammonium chloride-treated particles and NOIPs suggests either that the NOIPs have not undergone any conformational change during maturation or that the conformational change is reversible. Comparison with the cryo-EM reconstruction of immature dengue virus established the locations of the N-linked glycosylation sites of these viruses, verifying the interpretation of the reconstructions of the immature flaviviruses.

PubMed: 17376919
DOI: 10.1128/JVI.00037-07

Experimental method

ELECTRON MICROSCOPY (24 Å)