2OBE

Crystal Structure of Chimpanzee Adenovirus (Type 68/Simian 25) Major Coat Protein Hexon

2OBE の概要

• エントリーDOI: 10.2210/pdb2obe/pdb
• 関連するPDBエントリー: 1HX6 1P2Z 1P30 2INY
• 分子名称: Hexon protein, DIHYDROGENPHOSPHATE ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: trimer, double-barrel subunit, viral jellyroll, base, hypervariable tower, viral protein
• 由来する生物種: Simian adenovirus 25
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 319647.74

構造登録者

Xue, F.,Rux, J.J.,Burnett, R.M. (登録日: 2006-12-18, 公開日: 2007-07-24, 最終更新日: 2024-11-06)

主引用文献

Pichla-Gollon, S.L.,Drinker, M.,Zhou, X.,Xue, F.,Rux, J.J.,Gao, G.-P.,Wilson, J.M.,Ertl, H.C.J.,Burnett, R.M.,Bergelson, J.M.
Structure-based identification of a major neutralizing site in an adenovirus hexon
J.Virol., 81:1680-1689, 2007

PubMed Abstract: Virus-specific neutralizing antibodies present an obstacle to the effective use of adenovirus vectors for gene therapy and vaccination. The specific sites recognized by neutralizing antibodies have not been identified for any adenovirus, but they have been proposed to reside within the hexon, in small regions of the molecule that are exposed on the capsid surface and possess sequences that vary among serotypes. We have mapped the epitopes recognized by a panel of seven hexon-specific monoclonal antibodies that neutralize the chimpanzee adenovirus 68 (AdC68). Surface plasmon resonance experiments revealed that the antibodies compete for a single hexon binding site, and experiments with synthetic peptides indicated that this site resides within just one small surface loop. Mutations within this loop (but not in other surface loops) permitted virus to escape neutralization by all seven monoclonal antibodies and to resist neutralization by polyclonal antisera obtained from animals immunized against AdC68. These results indicate that a single small surface loop defines a major neutralization site for AdC68 hexon.

PubMed: 17108028
DOI: 10.1128/JVI.02023-06

実験手法

X-RAY DIFFRACTION (2.1 Å)