2OB9

Structure of bacteriophage HK97 tail assembly chaperone

Summary for 2OB9

• Entry DOI: 10.2210/pdb2ob9/pdb
• Descriptor: Tail assembly chaperone (2 entities in total)
• Functional Keywords: bacteriophage hk97, morphogenesis, tail assembly chaperone, chaperone
• Biological source: Enterobacteria phage HK97
• Total number of polymer chains: 2
• Total formula weight: 29474.43

Authors

McGrath, T.E.,Tuite, A.,Bona, D.,Saridakis, V.,Edwards, A.M.,Maxwell, K.,Chirgadze, N.Y. (deposition date: 2006-12-18, release date: 2007-12-18, Last modification date: 2024-10-16)

Primary citation

Pell, L.G.,Cumby, N.,Clark, T.E.,Tuite, A.,Battaile, K.P.,Edwards, A.M.,Chirgadze, N.Y.,Davidson, A.R.,Maxwell, K.L.
A conserved spiral structure for highly diverged phage tail assembly chaperones.
J.Mol.Biol., 425:2436-2449, 2013

PubMed Abstract: Tail assembly chaperones (TACs) are a family of proteins likely required for the morphogenesis of all long-tailed phages. In this study, we determined the crystal structure of gp13, the TAC of phage HK97. This structure is similar to that of the TAC from the Lactococcus phage p2 and two unannotated structures of likely TACs encoded in prophage-derived regions of Bacillus subtilis and Bacillus stearothermophilus. Despite the high sequence divergence of these proteins, gp13 forms a ring structure with similar dimensions to the spirals observed in the crystal lattices of these other proteins. Remarkably, these similar quaternary structures are formed through very different interprotomer interactions. We present functional data supporting the biological relevance of these spiral structures and propose that spiral formation has been the primary requirement for these proteins during evolution. This study presents an unusual example of diverged protein sequences and oligomerization mechanisms in the presence of conserved quaternary structure.

PubMed: 23542344
DOI: 10.1016/j.jmb.2013.03.035

Experimental method

X-RAY DIFFRACTION (2.3 Å)