2OB1

ppm1 with 1,8-ANS

2OB1 の概要

• エントリーDOI: 10.2210/pdb2ob1/pdb
• 関連するPDBエントリー: 2OB2
• 分子名称: Leucine carboxyl methyltransferase 1, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: ppm1 in the presence of 1, 8-ans, transferase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 110151.82

構造登録者

Groves, M.R. (登録日: 2006-12-18, 公開日: 2007-01-30, 最終更新日: 2023-08-30)

主引用文献

Groves, M.R.,Muller, I.B.,Kreplin, X.,Muller-Dieckmann, J.
A method for the general identification of protein crystals in crystallization experiments using a noncovalent fluorescent dye.
Acta Crystallogr.,Sect.D, 63:526-535, 2007

PubMed Abstract: A technique is described whereby the addition of low concentrations (millimolar to micromolar) of the fluorescent dye 1,8-ANS to the protein solution prior to crystallization results in crystallization experiments in which protein crystals are strongly contrasted above background artifacts when exposed to low-intensity UV radiation. As 1,8-ANS does not covalently modify the protein sample, no further handling or purification steps are necessary. The system has been tested on a wide variety of protein samples and it has been shown that the addition of 1,8-ANS has no discernible effect on the crystallization frequencies or crystallization conditions of these proteins. As 1,8-ANS interacts with a wide variety of proteins, this is proposed to be a general solution for the automated classification of protein crystallization images and the detection of protein crystals. The results also demonstrate the expected discrimination between salt and protein crystals, as well as allowing the straightforward identification of small crystals that grow in precipitate or under a protein skin.

PubMed: 17372358
DOI: 10.1107/S0907444906056137

実験手法

X-RAY DIFFRACTION (1.9 Å)