2O8L

Structure of V8 protease from staphylococcus aureus

2O8L の概要

• エントリーDOI: 10.2210/pdb2o8l/pdb
• 関連するPDBエントリー: 1QY6
• 分子名称: V8 protease, POTASSIUM ION (3 entities in total)
• 機能のキーワード: serine protease; enzyme, hydrolase
• 由来する生物種: Staphylococcus aureus
• 細胞内の位置: Secreted : Q99V45
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29714.79

構造登録者

Delbaere, L.T.J.,Prasad, L.,Leduc, Y. (登録日: 2006-12-12, 公開日: 2007-01-02, 最終更新日: 2023-08-30)

主引用文献

Prasad, L.,Leduc, Y.,Hayakawa, K.,Delbaere, L.T.J.
The Structure of a Universally Employed Enazyme: V8 Protease from Staphylococcus Aureus
Acta Crystallogr.,Sect.D, 60:256-259, 2004

PubMed Abstract: V8 protease, an extracellular protease of Staphylococcus aureus, is related to the pancreatic serine proteases. The enzyme cleaves peptide bonds exclusively on the carbonyl side of aspartate and glutamate residues. Unlike the pancreatic serine proteases, V8 protease possesses no disulfide bridges. This is a major evolutionary difference, as all pancreatic proteases have at least two disulfide bridges. The structure of V8 protease shows structural similarity with several other serine proteases, specifically the epidermolytic toxins A and B from S. aureus and trypsin, in which the conformation of the active site is almost identical. V8 protease is also unique in that the positively charged N-terminus is involved in determining the substrate-specificity of the enzyme.

PubMed: 14747701
DOI: 10.1107/S090744490302599X

実験手法

X-RAY DIFFRACTION (1.5 Å)