2O8C

human MutSalpha (MSH2/MSH6) bound to ADP and an O6-methyl-guanine T mispair

Summary for 2O8C

• Entry DOI: 10.2210/pdb2o8c/pdb
• Related: 2O8B 2O8D 2O8E 2O8F
• Descriptor: 5'-D(*GP*AP*AP*CP*CP*GP*CP*(6OG)P*CP*GP*CP*TP*AP*GP*G)-3', 5'-D(*CP*CP*TP*AP*GP*CP*GP*TP*GP*CP*GP*GP*TP*TP*C)-3', DNA mismatch repair protein Msh2, ... (7 entities in total)
• Functional Keywords: dna mismatch repair, dna damage response, protein-dna complex, dna mispair, cancer, o6-methyl-guanine, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus (Potential): P43246; Nucleus: P52701
• Total number of polymer chains: 4
• Total formula weight: 231032.54

Authors

Warren, J.J.,Pohlhaus, T.J.,Changela, A.,Modrich, P.L.,Beese, L.S. (deposition date: 2006-12-12, release date: 2007-06-05, Last modification date: 2023-08-30)

Primary citation

Warren, J.J.,Pohlhaus, T.J.,Changela, A.,Iyer, R.R.,Modrich, P.L.,Beese, L.S.
Structure of the Human MutSalpha DNA Lesion Recognition Complex.
Mol.Cell, 26:579-592, 2007

PubMed Abstract: Mismatch repair (MMR) ensures the fidelity of DNA replication, initiates the cellular response to certain classes of DNA damage, and has been implicated in the generation of immune diversity. Each of these functions depends on MutSalpha (MSH2*MSH6 heterodimer). Inactivation of this protein complex is responsible for tumor development in about half of known hereditary nonpolyposis colorectal cancer kindreds and also occurs in sporadic tumors in a variety of tissues. Here, we describe a series of crystal structures of human MutSalpha bound to different DNA substrates, each known to elicit one of the diverse biological responses of the MMR pathway. All lesions are recognized in a similar manner, indicating that diversity of MutSalpha-dependent responses to DNA lesions is generated in events downstream of this lesion recognition step. This study also allows rigorous mapping of cancer-causing mutations and furthermore suggests structural pathways for allosteric communication between different regions within the heterodimer.

PubMed: 17531815
DOI: 10.1016/j.molcel.2007.04.018

Experimental method

X-RAY DIFFRACTION (3.37 Å)