2O88

Crystal structure of the N114A mutant of ABL-SH3 domain complexed with a designed high-affinity peptide ligand: implications for SH3-ligand interactions

2O88 の概要

• エントリーDOI: 10.2210/pdb2o88/pdb
• 関連するPDBエントリー: 1BBZ
• 分子名称: Proto-oncogene tyrosine-protein kinase ABL1, P41 peptide, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: sh3 domain high affinity peptide complex, signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm, cytoskeleton. Isoform IB: Nucleus membrane; Lipid-anchor: P00519
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 15022.53

構造登録者

Camara-Artigas, A. (登録日: 2006-12-12, 公開日: 2007-05-01, 最終更新日: 2024-10-30)

主引用文献

Camara-Artigas, A.,Palencia, A.,Martinez, J.C.,Luque, I.,Gavira, J.A.,Garcia-Ruiz, J.M.
Crystallization by capillary counter-diffusion and structure determination of the N114A mutant of the SH3 domain of Abl tyrosine kinase complexed with a high-affinity peptide ligand.
Acta Crystallogr.,Sect.D, 63:646-652, 2007

PubMed Abstract: The recognition of proline-rich ligands by SH3 domains is part of the process leading to diseases such as cancer or AIDS. Understanding the molecular determinants of the binding affinity and specificity of these interactions is crucial for the development of potent inhibitors with therapeutic potential. In this study, the crystallographic structure of the N114A mutant of the SH3 domain of the Abelson leukaemia virus tyrosine kinase complexed with a high-affinity peptide is presented. The crystallization was carried out using the capillary counter-diffusion technique, which facilitates the screening, manipulation and transport of the crystals and allows the collection of X-ray data directly from the capillary in which the crystals were grown. The crystals of the N114A mutant belong to the orthorhombic P2(1)2(1)2(1) space group, with unit-cell parameters a = 48.2, b = 50.1, c = 56.4 A. The quality of the diffraction data set has allowed the structure of the complex to be determined at a resolution limit of 1.75 A.

PubMed: 17452790
DOI: 10.1107/S0907444907011109

実験手法

X-RAY DIFFRACTION (1.75 Å)