2O5P

Crystal structure of the full length ferric pyoverdine outer membrane receptor FpvA of Pseudomonas aeruginosa in its apo form

Summary for 2O5P

• Entry DOI: 10.2210/pdb2o5p/pdb
• Related: 1XKH 2iah
• Descriptor: Ferripyoverdine receptor, PHOSPHATE ION, 3,6,9,12,15-PENTAOXATRICOSAN-1-OL (3 entities in total)
• Functional Keywords: fpva, pyoverdine, pseudomonas, cobessi, transport protein
• Biological source: Pseudomonas aeruginosa
• Cellular location: Cell outer membrane: P48632
• Total number of polymer chains: 2
• Total formula weight: 174412.37

Authors

Cobessi, D. (deposition date: 2006-12-06, release date: 2007-10-30, Last modification date: 2023-08-30)

Primary citation

Brillet, K.,Journet, L.,Celia, H.,Paulus, L.,Stahl, A.,Pattus, F.,Cobessi, D.
A beta strand lock exchange for signal transduction in TonB-dependent transducers on the basis of a common structural motif.
Structure, 15:1383-1391, 2007

PubMed Abstract: Transport of molecules larger than 600 Da across the outer membrane involves TonB-dependent receptors and TonB-ExbB-ExbD of the inner membrane. The transport is energy consuming, and involves direct interactions between a short N-terminal sequence of receptor, called the TonB box, and TonB. We solved the structure of the ferric pyoverdine (Pvd-Fe) outer membrane receptor FpvA from Pseudomonas aeruginosa in its apo form. Structure analyses show that residues of the TonB box are in a beta strand which interacts through a mixed four-stranded beta sheet with the periplasmic signaling domain involved in interactions with an inner membrane sigma regulator. In this conformation, the TonB box cannot form a four-stranded beta sheet with TonB. The FhuA-TonB or BtuB-TonB structures show that the TonB-FpvA interactions require a conformational change which involves a beta strand lock-exchange mechanism. This mechanism is compatible with movements of the periplasmic domain deduced from crystallographic analyses of FpvA, FpvA-Pvd, and FpvA-Pvd-Fe.

PubMed: 17997964
DOI: 10.1016/j.str.2007.08.013

Experimental method

X-RAY DIFFRACTION (2.77 Å)