2O4V

An arginine ladder in OprP mediates phosphate specific transfer across the outer membrane

2O4V の概要

• エントリーDOI: 10.2210/pdb2o4v/pdb
• 分子名称: Porin P, CALCIUM ION, PHOSPHATE ION, ... (7 entities in total)
• 機能のキーワード: outer membrane, porin, phosphate, transport, channel, membrane protein
• 由来する生物種: Pseudomonas aeruginosa PAO1
• 細胞内の位置: Cell outer membrane; Multi-pass membrane protein: P05695
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 137689.39

構造登録者

Moraes, T.F.,Bains, M.,Hancock, R.E.,Strynadka, N.C. (登録日: 2006-12-05, 公開日: 2006-12-26, 最終更新日: 2023-12-27)

主引用文献

Moraes, T.F.,Bains, M.,Hancock, R.E.,Strynadka, N.C.
An arginine ladder in OprP mediates phosphate-specific transfer across the outer membrane.
Nat.Struct.Mol.Biol., 14:85-87, 2007

PubMed Abstract: The outer membrane protein OprP mediates the transport of essential phosphate anions into the pathogenic bacterium Pseudomonas aeruginosa. Here we report the crystallographic structure of trimeric OprP at 1.9-A resolution, revealing an unprecedented 9-residue arginine 'ladder' that spans from the extracellular surface down through a constriction zone where phosphate is coordinated. Lysine residues coat the inner periplasmic surface, creating an 'electropositive sink' that pulls the phosphates through the eyelet and into the cell.

PubMed: 17187075
DOI: 10.1038/nsmb1189

実験手法

X-RAY DIFFRACTION (1.94 Å)