2NXP

Structure of NTD2 domain of the human TAF5 subunit of TFIID

2NXP の概要

• エントリーDOI: 10.2210/pdb2nxp/pdb
• 分子名称: Transcription initiation factor TFIID subunit 5, CALCIUM ION (3 entities in total)
• 機能のキーワード: transcription factor, tfiid subunit, taf5, transcription
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q15542
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 151060.11

構造登録者

Bhattacharya, S.,Takada, S.,Jacobson, R.H. (登録日: 2006-11-17, 公開日: 2007-01-09, 最終更新日: 2024-11-20)

主引用文献

Bhattacharya, S.,Takada, S.,Jacobson, R.H.
Structural analysis and dimerization potential of the human TAF5 subunit of TFIID.
Proc.Natl.Acad.Sci.Usa, 104:1189-1194, 2007

PubMed Abstract: TFIID is an essential factor required for RNA polymerase II transcription but remains poorly understood because of its intrinsic complexity. Human TAF5, a 100-kDa subunit of general transcription factor TFIID, is an essential gene and plays a critical role in assembling the 1.2 MDa TFIID complex. We report here a structural analysis of the TAF5 protein. Our structure at 2.2-A resolution of the TAF5-NTD2 domain reveals an alpha-helical domain with distant structural similarity to RNA polymerase II CTD interacting factors. The TAF5-NTD2 domain contains several conserved clefts likely to be critical for TFIID complex assembly. Our biochemical analysis of the human TAF5 protein demonstrates the ability of the N-terminal half of the TAF5 gene to form a flexible, extended dimer, a key property required for the assembly of the TFIID complex.

PubMed: 17227857
DOI: 10.1073/pnas.0610297104

実験手法

X-RAY DIFFRACTION (2.17 Å)