2NXB
Crystal structure of human Bromodomain containing protein 3 (BRD3)
Summary for 2NXB
| Entry DOI | 10.2210/pdb2nxb/pdb |
| Descriptor | Bromodomain-containing protein 3, SODIUM ION, 1,2-ETHANEDIOL, ... (4 entities in total) |
| Functional Keywords | brd3, bromodomain containing protein 3, orfx, ring3 like gene, ring3l, structural genomics, structural genomics consortium, sgc, signaling protein |
| Biological source | Homo sapiens (human) |
| Cellular location | Nucleus : Q15059 |
| Total number of polymer chains | 2 |
| Total formula weight | 29631.15 |
| Authors | Filippakopoulos, P.,Bullock, A.,Cooper, C.,Keates, K.,Berridge, G.,Pike, A.,Bunkoczi, G.,Edwards, A.,Arrowsmith, C.,Sundstrom, M.,von Delft, F.,Knapp, S.,Structural Genomics Consortium (SGC) (deposition date: 2006-11-17, release date: 2006-12-12, Last modification date: 2023-08-30) |
| Primary citation | Filippakopoulos, P.,Picaud, S.,Mangos, M.,Keates, T.,Lambert, J.P.,Barsyte-Lovejoy, D.,Felletar, I.,Volkmer, R.,Muller, S.,Pawson, T.,Gingras, A.C.,Arrowsmith, C.H.,Knapp, S. Histone recognition and large-scale structural analysis of the human bromodomain family. Cell(Cambridge,Mass.), 149:214-231, 2012 Cited by PubMed Abstract: Bromodomains (BRDs) are protein interaction modules that specifically recognize ε-N-lysine acetylation motifs, a key event in the reading process of epigenetic marks. The 61 BRDs in the human genome cluster into eight families based on structure/sequence similarity. Here, we present 29 high-resolution crystal structures, covering all BRD families. Comprehensive crossfamily structural analysis identifies conserved and family-specific structural features that are necessary for specific acetylation-dependent substrate recognition. Screening of more than 30 representative BRDs against systematic histone-peptide arrays identifies new BRD substrates and reveals a strong influence of flanking posttranslational modifications, such as acetylation and phosphorylation, suggesting that BRDs recognize combinations of marks rather than singly acetylated sequences. We further uncovered a structural mechanism for the simultaneous binding and recognition of diverse diacetyl-containing peptides by BRD4. These data provide a foundation for structure-based drug design of specific inhibitors for this emerging target family. PubMed: 22464331DOI: 10.1016/j.cell.2012.02.013 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.4 Å) |
Structure validation
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