2NUI

X-ray Structure of synthetic [D83A]RNase A

2NUI の概要

• エントリーDOI: 10.2210/pdb2nui/pdb
• 分子名称: Ribonuclease pancreatic, CHLORIDE ION, 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• 機能のキーワード: synthetic protein, hydrolase
• 由来する生物種: Bos taurus
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13979.92

構造登録者

Boerema, D.J.,Tereshko, V.A.,Kent, S.B.H. (登録日: 2006-11-09, 公開日: 2007-10-30, 最終更新日: 2024-11-06)

主引用文献

Boerema, D.J.,Tereshko, V.A.,Kent, S.B.
Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A
Biopolymers, 90:278-286, 2008

PubMed Abstract: The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution.

PubMed: 17610259
DOI: 10.1002/bip.20800

実験手法

X-RAY DIFFRACTION (1.1 Å)