2NTX

Prone8

2NTX の概要

• エントリーDOI: 10.2210/pdb2ntx/pdb
• 関連するPDBエントリー: 2NTY
• 分子名称: Emb|CAB41934.1 (2 entities in total)
• 機能のキーワード: dimer, guanine nucleotide exchange factor, signaling protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84345.79

構造登録者

Thomas, C.,Fricke, I.,Scrima, A.,Berken, A.,Wittinghofer, A. (登録日: 2006-11-08, 公開日: 2007-01-23, 最終更新日: 2024-11-13)

主引用文献

Thomas, C.,Fricke, I.,Scrima, A.,Berken, A.,Wittinghofer, A.
Structural Evidence for a Common Intermediate in Small G Protein-GEF Reactions
Mol.Cell, 25:141-149, 2007

PubMed Abstract: Rho of plants (Rop) proteins belong to the superfamily of small GTP-binding (G) proteins and are vital regulators of signal transduction in plants. In order to become activated, Rop proteins need to exchange GDP for GTP, an intrinsically slow process catalyzed by guanine nucleotide exchange factors (GEFs). RopGEFs show no homology to animal RhoGEFs, and the catalytic mechanism remains elusive. GEF-catalysed nucleotide exchange proceeds via transient ternary and stable binary complexes. While a number of structural studies have analyzed binary nucleotide-free G protein-GEF complexes, very little is known about the ternary complexes. Here we report the X-ray structure of the catalytic PRONE domain of RopGEF8 from Arabidopsis thaliana, both alone and in a ternary complex with Rop4 and GDP. The features of the latter complex, a transient intermediate of the exchange reaction never directly observed before, suggest a common mechanism of catalyzed nucleotide exchange applicable to small G proteins in general.

PubMed: 17218277
DOI: 10.1016/j.molcel.2006.11.023

実験手法

X-RAY DIFFRACTION (2.2 Å)