2NPS
Crystal Structure of the Early Endosomal SNARE Complex
Summary for 2NPS
| Entry DOI | 10.2210/pdb2nps/pdb |
| Descriptor | Vesicle-associated membrane protein 4, Syntaxin 13, Vesicle transport through interaction with t-SNAREs homolog 1A, ... (5 entities in total) |
| Functional Keywords | vesicle fusion, snare complex, early endosomal snare complex, syntaxin 6, syntaxin 13, vti1a, vamp4, transport protein |
| Biological source | Mus musculus (house mouse) More |
| Cellular location | Golgi apparatus, trans-Golgi network membrane ; Single-pass type IV membrane protein : O70480 Membrane ; Single-pass type IV membrane protein : Q9JI51 Golgi apparatus membrane ; Single-pass type IV membrane protein : O43752 |
| Total number of polymer chains | 4 |
| Total formula weight | 35507.08 |
| Authors | Zwilling, D.,Cypionka, A.,Pohl, W.H.,Fasshauer, D.,Walla, P.J.,Wahl, M.C.,Jahn, R. (deposition date: 2006-10-30, release date: 2006-12-26, Last modification date: 2023-10-25) |
| Primary citation | Zwilling, D.,Cypionka, A.,Pohl, W.H.,Fasshauer, D.,Walla, P.J.,Wahl, M.C.,Jahn, R. Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies Embo J., 26:9-18, 2007 Cited by PubMed Abstract: SNARE proteins mediate membrane fusion in eukaryotic cells. They contain conserved SNARE motifs that are usually located adjacent to a C-terminal transmembrane domain. SNARE motifs spontaneously assemble into four helix bundles, with each helix belonging to a different subfamily. Liposomes containing SNAREs spontaneously fuse with each other, but it is debated how the SNAREs are distributed between the membranes. Here, we report that the SNAREs mediating homotypic fusion of early endosomes fuse liposomes in five out of seven possible combinations, in contrast to previously studied SNAREs involved in heterotypic fusion events. The crystal structure of the early endosomal SNARE complex resembles that of the neuronal and late endosomal complexes, but differs in surface side-chain interactions. We conclude that homotypic fusion reactions may proceed with multiple SNARE topologies, suggesting that the conserved SNARE structure allows for flexibility in the initial interactions needed for fusion. PubMed: 17159904DOI: 10.1038/sj.emboj.7601467 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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