2NPS

Crystal Structure of the Early Endosomal SNARE Complex

2NPS の概要

• エントリーDOI: 10.2210/pdb2nps/pdb
• 分子名称: Vesicle-associated membrane protein 4, Syntaxin 13, Vesicle transport through interaction with t-SNAREs homolog 1A, ... (5 entities in total)
• 機能のキーワード: vesicle fusion, snare complex, early endosomal snare complex, syntaxin 6, syntaxin 13, vti1a, vamp4, transport protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Golgi apparatus, trans-Golgi network membrane ; Single-pass type IV membrane protein : O70480; Membrane ; Single-pass type IV membrane protein : Q9JI51; Golgi apparatus membrane ; Single-pass type IV membrane protein : O43752
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 35507.08

構造登録者

Zwilling, D.,Cypionka, A.,Pohl, W.H.,Fasshauer, D.,Walla, P.J.,Wahl, M.C.,Jahn, R. (登録日: 2006-10-30, 公開日: 2006-12-26, 最終更新日: 2023-10-25)

主引用文献

Zwilling, D.,Cypionka, A.,Pohl, W.H.,Fasshauer, D.,Walla, P.J.,Wahl, M.C.,Jahn, R.
Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies
Embo J., 26:9-18, 2007

PubMed Abstract: SNARE proteins mediate membrane fusion in eukaryotic cells. They contain conserved SNARE motifs that are usually located adjacent to a C-terminal transmembrane domain. SNARE motifs spontaneously assemble into four helix bundles, with each helix belonging to a different subfamily. Liposomes containing SNAREs spontaneously fuse with each other, but it is debated how the SNAREs are distributed between the membranes. Here, we report that the SNAREs mediating homotypic fusion of early endosomes fuse liposomes in five out of seven possible combinations, in contrast to previously studied SNAREs involved in heterotypic fusion events. The crystal structure of the early endosomal SNARE complex resembles that of the neuronal and late endosomal complexes, but differs in surface side-chain interactions. We conclude that homotypic fusion reactions may proceed with multiple SNARE topologies, suggesting that the conserved SNARE structure allows for flexibility in the initial interactions needed for fusion.

PubMed: 17159904
DOI: 10.1038/sj.emboj.7601467

実験手法

X-RAY DIFFRACTION (2.5 Å)