2NLU

Domain-Swapped Dimer of the PWWP Module of Human Hepatoma-derived Growth Factor

2NLU の概要

• エントリーDOI: 10.2210/pdb2nlu/pdb
• 関連するPDBエントリー: 1N27 1RI0 2B8A
• NMR情報: BMRB: 5902
• 分子名称: Hepatoma-derived growth factor (1 entity in total)
• 機能のキーワード: hdgf, hhdgf, hrp, hath, pwwp, heparin, domain-swapping, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P51858
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 23128.24

構造登録者

Sue, S.C.,Lee, W.T.,Huang, T.H. (登録日: 2006-10-20, 公開日: 2007-09-04, 最終更新日: 2023-12-27)

主引用文献

Sue, S.C.,Lee, W.T.,Tien, S.C.,Lee, S.C.,Yu, J.G.,Wu, W.J.,Wu, W.G.,Huang, T.H.
PWWP module of human hepatoma-derived growth factor forms a domain-swapped dimer with much higher affinity for heparin
J.Mol.Biol., 367:456-472, 2007

PubMed Abstract: Hepatoma-derived growth factor (hHDGF)-related proteins (HRPs) comprise a new growth factor family sharing a highly conserved and ordered N-terminal PWWP module (residues 1-100, previously referred to as a HATH domain) and a variable disordered C-terminal domain. We have shown that the PWWP module is responsible for heparin binding and have solved its structure in solution. Here, we show that under physiological conditions, both the PWWP module and hHDGF can form dimers. Surface plasmon resonance (SPR) studies revealed that the PWWP dimer binds to heparin with affinity that is two orders of magnitude higher (K(d)=13 nM) than that of the monomeric PWWP module (K(d)=1.2 microM). The monomer-dimer equilibrium properties and NMR structural data together suggest that the PWWP dimer is formed through a domain-swapping mechanism. The domain-swapped PWWP dimer structures were calculated on the basis of the NMR data. The results show that the two PWWP protomers exchange their N-terminal hairpin to form a domain-swapped dimer. The two monomers in a dimer are linked by the long flexible L2 loops, a feature supported by NMR relaxation data for the monomer and dimer. The enhanced heparin-binding affinity of the dimer can be rationalized in the framework of the dimer structure.

PubMed: 17270212
DOI: 10.1016/j.jmb.2007.01.010

実験手法

SOLUTION NMR